61222
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- 中文名稱: 谷氨酰胺合成酶
- 英文名稱: Glutamine Synthetase
The enzyme is useful for the determination of ammonia and ATP in clinical analysis.
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- 產(chǎn)品類別: 酶
- 生化診斷: 比色法
規(guī)格
| Appearance | white to light yellow lyophilizate |
| Activity | ≧15.0 U/mg |
| Contaminants | Catalase ≦0.5 U/U％ |
| Stabilizer | sucrose |
| Storage condition | below-20℃ |
特性
| Molecular weight | ca. 900 kDa (gel filtration) |
| Structure | 57 kDa (SDS-PAGE) |
| Isoelectric point | 6.5 |
| Michaelis constant | 1.5×10-2M (L-glutamate) 1.3×10-4M (ammonia) 8.7×10-4M (ATP) |
| pH Optimum | ca. 7.0 |
| pH Stability | 6.5–9.5 |
| Optimum temperature | 60℃ |
| Thermal stability | below 40℃ |
| Inhibitors | methionine sulfoximine, carbamyl phosphate |
| Activators | Mg2+，Mn2+ |
| Specificity | L-glutamate (100), D-glutamate (0.8) NH?(100), NH?OH (12) ATP (100), GTP (2.5) |
應(yīng)用
The enzyme is useful for the determination of ammonia and ATP in clinical analysis.
參考文獻(xiàn)
Ebner, E. et al., “Methods in Enzymology,” Vol. 17A, Academic Press, New York, 1970, pp. 910–922.
